血塞通对AMI后心肌重塑及TGF-β1/Smads通路的干预研究

Effect of Xuesaitong Soft Capsules in intervening myocardial remodeling and TGF-β1/Smads after AMI

目的通过研究血塞通在大鼠急性心梗后心肌重塑中的干预作用及对TGF-β1/Smads通路的影响,探讨血塞通对急性心梗后心肌重塑的效应机制,为血塞通治疗急性心梗后心肌重塑提供实验依据。方法清洁级Wistar雄性大鼠50只随机分为5组,分别为正常组、模型组、假手术组、血塞通组、基质细胞衍生因子-1受体阻断剂(AMD3100)组,每组10只。血塞通组大鼠术前3天给药,1次/d。7 d后,实时荧光定量RT-PCR检测心肌组织转化生长因子(TGF)β1mRNA、Smad3mRNA、Smad7mRNA表达,分析各组非梗死区心肌胶原含量,并观察心肌组织HE染色结果。结果与假手术组相比较,模型组心肌组织TGF-β1mRNA、Smad3mRNA表达均增加(P<0.05),而Smad7mRNA表达降低(P<0.05)。与模型组相比较,血塞通组能降低心肌组织TGF-β1mRNA、Smad3mRNA表达(P<0.05),增加Smad7mRNA表达(P<0.05),降低心肌胶原含量,并能减轻心肌组织的病理损伤。血塞通组与AMD3100组相比较,TGF-β1mRNA、Smad3mRNA、Smad7mRNA表达均无明显差异(P>0.05)。结论血塞通对急性心梗后心肌重塑有一定抑制作用。

Objective Through studying the effect of Xuesaitong Soft Capsules （Xuesaitong） in intervening myocardial remodeling in rats with acute myocardial infraction （AMI） and its influence on TGF-[M/Smads, to discuss the mechanism of Xuesaitong in intervening myocardial_ remodeling after AMI, and provide experimental basis for treating myocardial remodeling after AMI with Xuesaitong Soft Capsules. Methods Male Wistar rats （n = 50, specific pathogen free） were randomly divided into normal group, model group, sham-operation group, Xuesaitong group and AMD3100 group （each n = 10）. Xuesaitong group were given medicine once a day 3 days before operation. After 7 days, the expressions of TGFq31 mRNA, Smad3 mRNA and Smad7 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction （RT-PCR）, collagen content was analyzed in non- infracted myocardial tissue in all groups, and myocardial tissue was observed by applying HE staining. Results The expressions of TGF-β1 mRNA and Smad3 mRNA increased （P 〈 0.05 ）, and expression of Smad7 mRNA decreased （P 〈 0.05） in model group compared with those in sham-operation group. The expressions of TGF-151 mRNA and Smad3 mRNA decreased （ P 〈 0.05 ）, expression of Smad7 mRNA increased （P 〈 0.05 ）, myocardial collagen content decreased, and myocardial pathological lesion was alleviated in Xuesaitong group compared with those in model group. There were no significant differences in expressions of TGF-β1 mRNA, Smad3 mRNA and Smad7 mRNA between Xuesaitong group and AMD3100 group （ P 〉 0.05 ）. Conclusion Xuesaitong has an inhibitory effect on myocardial remodeling after AMI.