化学发光法测定天麻素

Determination of Gastrodin with the Method of Chemilumincsccncc

天麻为兰科天麻属植物天麻Gastrodia elata Blume的块茎,性味甘平,有祛风定惊之功效,以天麻素为有效成分应用于临床。对天麻素的测定多用薄层层析-紫外分光光度法,而用化学发光法却未见报道。本文使用鲁米诺-过氧化氢-高锰酸钾发光体系,对天麻素进行了定量分析,取得了良好的效果。

Using Luminol-H2O2-KMnO4 as the Luminescent system and H2O2 generated from the hydrolysis and enzymatic reaction of gastrodin as the quantitative product, the luminescent intensity was measured. From the intensity of the product H2O2 the content of gastrodin has been calculated.