人乳头瘤病毒、疱疹病毒I型及人巨细胞病毒与口腔鳞癌关系的研究

A Study on the Association Among the Infections of Human Papillomavirus、Herpes Simplex Virus Type Ⅰ、Human Cytomegalivirus and Oral Sqramous Cell Carcinoma

目的 :研究口腔鳞癌与人乳头瘤病毒 (HPV)、疱疹病毒I型 (HSV -I)和人巨细胞病毒 (HCMV)的关系。方法 :采用斑点杂交和PCR技术检测 32例口腔鳞癌、14例口腔白斑和 10例正常口腔粘膜中HPV1 6 、HSV -I及HCMVDNA。结果 :在正常口腔粘膜、白斑及口腔鳞癌中HPV1 6 、HSV -I及HCMVDNA感染率分别为 0 %、35 7%、5 0 0 % ,40 0 %、5 0 0 %、43 3%和 0 %、14 3%、2 8 1% ,口腔鳞癌及白斑组织中HPV1 6 -DNA的检出率均高于正常口腔 ,且差别具有显著性 (p <0 0 5 ) ;但HSV -I和HCMVDNA在口腔疾患中的检出率与正常比较无显著差别 (p >0 0 5 )。结论 :HPV1 6 感染与口腔鳞癌的发生相关 ;HSV -I和HCMV可能参予口腔鳞癌的发生及发展 ,并且与HPV1 6

Objective:To demonstrate the presence of human papillomavirus(HPV) herpes simplex virus type I(HSV I)、human cytomegalovirus(HCMV)in oral spuamous cell carcinoma(OSCC).Methods:HPV 16 、HSV I and HCMVNDA sequence in normal oral tissue、leukoplakia and OSCC by dot hybridization and polymerase chain reaction were detected.[WT5”FZ]Results:It was showed that the positive rates of HPV 16 DNA in OSCC and leukoplakoa were significanly higher than that in normal oral tissue([WT5”BX]p<0 05) However there was no significance in the positive rates of HSV I and HCMVDNA between oral diseases and normal oral tissue(p>0.05).Conclusion:The results indicate that infection of HPV 16 may be related to the occurrence of OSCC;HSV I and HCMV may play an important role in the development of OSCC and probably enhance the oncogensis of HPV 16