人转铁蛋白-阿霉素结合物对耐阿霉素K562细胞的体外杀伤作用

Cytotoxicity of Transferrin-Adriamycin Conjugate to Adriamycin-Resistant K562 in Vitro

目的 探讨阿霉素 (ADM)与人转铁蛋白 (Tf)结合物对耐阿霉素 K5 6 2细胞株的体外杀伤作用。 方法 采用葡聚糖 (DEX) T- 10作为中介体、联接 Tf与 ADM制备结合物 Tf- ADM,用 MTT法比较结合物对耐ADM及 ADM敏感的 K5 6 2细胞的体外杀伤作用强度。 结果 结合物对耐 ADM的 K 5 6 2细胞株 72 h培养、MTT法测定结合物对耐 ADM的 K5 6 2细胞 IC5 0 为 4.9μmol,游离 ADM的 IC5 0 >17.2 4μmol,Tf对这两种细胞都没有体外杀伤作用。 结论 结合物 Tf- ADM可提高 ADM对耐 ADM的 K5 6 2细胞株的杀伤作用 ,此作用与 Tf无明显的关系。

Objective\ To investigate the cytotoxicity of transferrin\|adriamycin conjugate to adriamycin\|resistant K562 cells in vitro.\ Methods\ Human trasferrin(Tf) was selected to conjugate with adriamycin(ADM) via dextran T\|10 as liner to construct Tf\|ADM.\ Growth inhibition of adriamycin\|sensitive K562 cells and adriamycin\|resistant K562 cells was compared as evaluated by MTT\|assay.\ Results\ The IC\-\{50\} of conjugate was 4.9 μmol for adriamycin\|resistant K562 cells incubated for 72 h as determined by MTT\|assay,and the IC\-\{50\} of free ADM was more than 17.24 μmol. \ The cytotoxicity of Tf toward both adriamycin\|sensitive and adriamycin\|resistant K562 cells was lower.\ Conclusion\ The conjugate Tf\|ADM can increase the cytotoxicity effect toward K562/ADM cell lines,which is not Tf\|dependent. \;