细胞周期素D_1在结肠癌中的表达

Cyclin D_1 Expression in Colorectal Carcinoma

目的 探讨细胞周期素 ( cyclin) D1与 p53基因在结肠癌中的表达意义及其关系。 方法 应用原位杂交技术和免疫组织化学技术检测结肠癌中 cyclin D1基因 m RNA和 p53蛋白的表达。统计处理采用卡方检验。 结果 发现结肠癌组织和癌旁组织中 cyclin D1表达阳性率分别为 63 .4 % ( 40 / 63 )及 4 4 .4 4 % ( 2 8/ 63 ) ,两者之间差异有显著性 ( P<0 .0 5)。cyclin D1表达程度与癌组织分化程度及临床分期有关 ,差异有显著性 ( P<0 .0 5)。cyclin D1表达与其他临床病理指标无关 ( P>0 .0 5)。p53蛋白在结肠癌中阳性率为 3 8.0 9% ( 2 4 / 63 ) ,p53阳性的患者其 cyclinD1的表达较之 p53阴性者为高 ,差异有显著性 ( P<0 .0 5)。 结论 cyclin D1的过表达及 p53突变在结肠癌的癌变中可能起重要作用 ,cyclin D1的表达参与了癌细胞分化及发展过程。

Objective\ To investigate the expression of cyclin D\-1 gene in colorectal carcinoma and its relationship to p53 gene expression.\ Methods\ Hybridization in situ and immunohistochemical technigues were used.\ Results\ The positive rate in tumor tissue and nomal mucosa was 63 49% and 44 44% respectively,and was significantly different(P<0 05).\ The cyclin D\-1 expression intensity related to the tumor differenciation and pathological stage,but not to the depth of tumor invasion,lymph node metastasis.\ The p53 positivity was 38 09%.\ The cyclin D\-1 expression rate in p53 positive group was higher than that in p53 negative group(P<0 05).\ Conclusion\ The cyclin D\-1 overexpression and p53 mutation may be improtant in the carcinogenesis of colorectum,the expression of cyclin D\-1 participate in the tumor differenciation and progress.\;