地佐辛联合帕瑞昔布钠对疼痛小鼠行为学的影响

Influence of dezocine combined with parecoxib sodium on behavior of mice with pain

目的观察和评价地佐辛联合帕瑞昔布钠对急性疼痛小鼠镇痛作用的影响。方法将40只C57小鼠随机分为四组:空白对照组(A组)、地佐辛组(B组)、帕瑞昔布钠组(C组)、地佐辛联合帕瑞昔布钠组(D组),每组10只。各组分别给予腹腔注射无菌双蒸水0.2 mL;地佐辛5 mg/kg;帕瑞昔布钠10 mg/kg;地佐辛3 mg/kg联合帕瑞昔布钠10 mg/kg。每天1次,连续给药3 d,第3天给药15 min后,制作扭体法和甲醛疼痛模型,观察小鼠痛阈变化。结果与A组比较,其余各组小鼠扭体次数和舔足时间均明显减少,差异有统计学意义(P<0.05);与C组比较,B组小鼠明显减少了扭体次数和舔足时间,差异有统计学意义(P<0.05);D组小鼠扭体次数和舔后足时间显著少于B组,差异有统计学意义(P<0.05)。结论地佐辛和帕瑞昔布钠均可以有效缓解小鼠急性疼痛,地佐辛的镇痛作用较帕瑞昔布钠明显,而地佐辛联合帕瑞昔布钠能进一步增强镇痛效果,且可以减少地佐辛的用药量。

Objective To observe and evaluate the influence of dezocine combined with parecoxib sodium on the mice with acute pain. Methods 40 C57 mice were randomly divided into 4 groups：blank control group （group A）, dczocine group （group B ）, parecoxib sodium group （group C） and dczocine combined with parecoxib sodium group （group D）, 10 mice in each group. Group A received an intraperitoneal injection of 0.2 mL sterile double distilled water, Group B of 5 mg/kg dezocine ,group C of 10 mg/kg parecoxib sodium and group D of 3 mg/kg dezocine combined with 10 mg/kg parecoxib sodium ,once daily for 3 days of successive administration. On 3rd day, 15 minutes after administration ,writhing method and formalin pain model were ap- plied to observe the change of feeling threshold. Results Compared with group A ,the writhing times and licking paw times in other three groups decreased obviously with statistically significant difference （P〈0.05）. Compared with group C, the writhing times and licking paw times in group B decreased significantly with statistically significant difference（P〈0.05 ）. The writhing times and licking paw times in group D were prominently lower than those in group B （P〈0.05）. Conclusion Both dezocine and parecoxib sodium can effectively relieve acute pain, and the effect of dezocine is more obvious than parecoxib sodium. Moreover, dezocine combined with parecoxib sodium can further enhance the analgesic effect, and reduce the dosage of dezocine.