HPLC法同时测定偏瘫口服液中阿魏酸与丹酚酸B的含量

HPLC with dual- wavelength simultaneous determination of two effective ingredients in Piantan oral liquid by gradient elution

目的：利用双波长高效液相色谱法，建立偏瘫口服液中阿魏酸、丹酚酸B的含量测定方法。方法：采用双波长HPLC法，WatersSunFireC18（4．6mm（250mm，5μm）色谱柱，柱温35℃；甲醇-0．1％磷酸溶液为流动相，以1．0mL·min11流速梯度洗脱；阿魏酸检测波长为318nm、丹酚酸B检测波长为286nm。结果：根据回归方程，阿魏酸和丹酚酸B的线性范围分别为6．152—76．9μg（r=0．9998）、294．8～3685．0μg（r=1.0000）；加样回收率分别为99．7％（n=6）、98．2％（n=6）。结论：本方法操作简单，结果准确，重复性好，可有效地提高该制剂的内在质量，也为该药品标准进一步研究，提供一个准确的参考方法。

To establish an HPLC with dual - wavelength detection method for simultaneous determination of Ferulic acid and Salvi- anolic acid B in Piantan oral liquid. Methods： The quantitative analysis was carried out on a column of Waters SunFire C18 （4. 6 mm × 250 mm, 5 μm） by HPLC with dual - wavelength , by a gradient elution using methanol （A） and water （B） as mobile phase at a flow rate of 1. 0 mL . min-1 at 35 ℃ ; The eluate was detected by multiple wavelength： 318 nm for Ferulic acid （0 ~30min）, 286 nm for Salvianolic acid B （30 -50 min）. Results： The linear ranges of Ferulic acid and Salvianolic acid B fell within the ranges of 6. 152 - 76.91μg （ r = 0. 9998 ） and 294. 8 - 3685.0 μg （ r = 1. 0000） ; The average recoveries were 99. 7 % （ n = 6 ） for Ferulic acid and 98. 2% （ n =6） for Salvianolic acid B. Conclusion： This method is simple, accurate, reliable and can be use to improve inner quality, also provides a reference standard for the quality control of Piantan oral liquid.