摘要
ABEI{6-[N-(4-氨基丁基)-N-乙基]-氯基-2,3-二氢吩噻嗪-1,4-二酮}是化学发光免疫分析法(简称LIA)中最常用的发光标记试剂,但有关电化学发光免疫分析目前尚未见报道。本文利用自制的电化学发光仪,对ABEI和ABEI标记兔抗HCG的电化学发光行为进行了研究。提出了利用电化学发光进行免疫分析的新途径。
The electrogenerated chemiluminescence of ABEI and ABEI-HCG in H3BO3-KCl-Na2CO3 buffer solution at pH 11. 06 was studied. Co(Ⅱ)(200~600 μg/L) is capable of catalyzing the ABEI luminescence when a pulse potential is applied to the electrodes. Antibody of HCG is combined with ABEI to obtain ABEI-IgG. The detection limit of ABEI and ABEI-IgG are 2. 0 ×10-11mol/L and 2. 1×10-10 mol/L , respectively. The concentration of ABEI, from 1.0×10-10 to 5.0× 10-7 mol/ L is in a linear relationship with the intensity of light.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1991年第4期469-470,共2页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
电化学
发光法
发光标记试剂
ABEI
Electrogenerated chemiluminescent immunoassay, Aminobutyl ethylisoluminol, HCG
