毛细柱气相色谱法测定饲料中丙酸及丙酸盐

Detection of propanoic acid and propionate in feed by capillary gas chromatography

实验建立了气相色谱法测定饲料中丙酸及丙酸盐的分析方法。样品经盐酸酸化后，丙酸盐转化为丙酸，乙醚提取，毛细管柱（HP—FFAP，30m×0．32mm×0．25μm）分离，进行FID检测器检测。结果表明，方法的线性范围为5．0-500mg／L（R=0．9993），检出限为0．77mg／kg，丙酸的回收率为87.2％-93．4％，相对标准偏差为1．7％-3.2％。

A method based on capillary gas chromatography was developed to detect propanoic acid and propionate content in feed.Samples were acidified by hydrochloric acid ,propionate was turned into propanoie acid.Then samples were extracted with the diethyl ether, separated on a capillary column （HP-FFAP, 30 m×0.32 mm×0.25 μm）and deteted with a FID.The linear range of the determination was 5.0 - 500 mg/L and the linear correlation coefficient was 0.9993.The detec- tion limit of this method was 0.77 mg/kg.The recoveries ranged from 87.2% to 93.4% with RSD of 1.7% - 3.2%.