胰岛素对卵巢卵泡膜细胞黄体生成素/人绒毛膜促性腺激素受体表达的影响

Effect of insulin on the expression of luteinizing hormone human chorionic gonadotropin receptor in cultured porcine theca cells

目的 :研究胰岛素对猪卵巢卵泡膜细胞黄体生成素 /人绒毛膜促性腺激素 (luteinizinghormone/humanchorionicgonadotropin ,LH /hCG)受体表达的影响。 方法 :采用细胞免疫组化及Westernblot法分别检测LH /hCG受体的蛋白表达水平。结果 :细胞免疫组化结果显示促卵泡激素 (follicle stimulatinghormone,FSH) +黄体生成素(luteinizinghormone,LH)处理的细胞淡黄色着色且阳性细胞数少 ;而FSH +LH +胰岛素 (insulin ,INS)处理的细胞为深黄色着色 ,阳性细胞数多 ,FSH +LH +INS组光密度 (0 .35 3± 0 .0 13)与FSH +LH组 (0 .2 87± 0 .0 0 9)相比LH/hCG受体表达明显增强 ,差异有显著性 ,P <0 .0 1。Westernblot结果显示FSH +LH +INS组 (6 5 98± 388)LH/hCG受体蛋白分别在 6× 10 3、5× 10 3、3.5× 10 3 出现条带 ,而FSH +LH组 (2 2 37± 395 )仅在 6× 10 3 处出现条带 ,两组比较 ,差异有显著性 ,P <0 .0 1。结论 :胰岛素可以增强卵泡膜细胞LH /hCG受体的表达 。

Objective: To study the effect of insulin on the expression of luteinizing hormone human chorionic gonadotropin receptor(LH/hCG receptor) of cultured porcine theca cells and look into the possible mechanism of the arrest of follicular growth in polycystic ovary syndrome(POS). Methods: The primary model of cultured porcine theca cells in vitro was established and the cells were treated differently. The level of LH/hCG receptor in the cells was checked by Western blot and cell immuno histochemistry methods. Results: The level of LH/hCG receptor in the cells of group FSH+LH+INS(6598±388)(0.353±0.013) was significantly higher than that of group FSH+LH(2237±395) (0.287 ±0.009), P <0.01 by Western blot and cell immunohistochemistry. The expression of LH/hCG receptor in the cells of group FSH+LH+INS showed 6×10 3, 5×10 3, 3.5×10 3 while group FSH+LH showed only 6×10 3. Conclusion: Insulin enhanced the expression of LH/hCG receptor of cultured porcine theca cells. Hyperinsulinemia may enhance the expression of LH/hCG receptor so as to lose the regularing during menstrical period and probably the arrest of follicular growth in patients with PCOS implicated with hyperinsulinemia.