老年大鼠肺泡Ⅱ型细胞细胞外基质成分和基底膜厚度的变化

Changes of extracellular matrix in type Ⅱ cells and thickness of basement membrane in old rats

目的 :探讨老年大鼠肺泡Ⅱ型细胞细胞外基质成分和基底膜厚度的变化。方法 :用免疫细胞化学和图像分析技术分析幼年 (1～ 2个月 )、中年 (8～ 9个月 )和老年 (2 5～ 2 8个月 )大鼠肺泡Ⅱ型细胞层粘连蛋白和纤维连接蛋白的变化及肺泡Ⅱ型细胞基底膜的厚度。结果 :老年大鼠肺泡Ⅱ型细胞层粘连蛋白减少 ,纤维连接蛋白增多 ,基底膜增厚。结论 :老年大鼠肺泡Ⅱ型细胞细胞外基质的代谢发生改变 。

Objective: To observe the changes of extracellular matrix in type Ⅱ cells and thickness of basement membrane beneath type Ⅱ cells in old rats. Methods: Inmmunocytochemistry and image analysis were used to measure the relative contents of laminin and fibronectin in type Ⅱ cells in young (1 2 months), adult (8 9 months) and old (25 28 months) rats. Results: There were less laminin, more fibronectin in type Ⅱ cells and thicker basement membrane beneath type Ⅱ cells in old rats. Conclusion: These results suggest the altered metabolism of extracellular matrix from type Ⅱ cells in old rats, which may result in abnormal recovery of alveoli after being injured.