PA感染与定植中血液PEA变化的研究

Study on PEA in the Blood in Detection and Differential Diagnosis of PA Infection and Colonization

目的探讨铜绿假单胞菌外毒素(PEA)在铜绿假单胞菌肺部感染及定植表达差异的临床价值。方法 SD大鼠180只,随机分为PA(铜绿假单胞菌)感染模型组,PA咽部定植组及生理盐水对照组。观察PA感染与定植中血液PEA变化。结果肺组织结构正常,定植组与对照组无明显区别,模型组出现了明显肺泡及肺间质水肿、出血、炎性细胞聚集的炎症表现。模型组血液PEA DNA表达在接种后第3、7、11天均明显高于定植组,二者比较存在统计学意义(P均<0.01)。结论血清检测有助于对铜绿假单胞菌感染与定植的鉴别诊断,且可预测肺部炎症的程度及预后。

Objective To investigate the pseudomonas aeruginosa exotoxin （PEA） in pseudomonas aeruginosa lung infection, and the clinical value of engraftment expression differences. Methods 180 SD rats, were randomly divided into PA（pseudomonas aeruginosa） infection model group, PA pharyngeal engraftment group and normal saline control group. Observation of PA infection and engraftment of PEA changes of blood. Results The structure of normal lung tissue, engraftment group and the control group, no significant difference between model group and there is an obvious alveolar pulmonary interstitial edema, hemorrhage and inflammatory cell aggregation of inflammation. Model group blood PEA DNA expression in 3, 7, 11 days after inoculation were significantly higher than that of engraftment group, which shows there is statistical signific.ance（P〈0.01）. Conclusion Serum helps to pseudomonas aeruginosa infection and engraftment, the differential diagnosis of the degree and the prognosis of lung inflammation and predictable.