MicroRNA-429表达与肝细胞癌预后的关系研究

The association between microRNA-429 expression and Hepatocellular carcinoma prognosis

摘要：目的探讨MicroRNA--429（miR--429）在肝细胞癌（Hepatocellularcarcinoma，HCC）中的表达情况及其与HCC临床病理特征和预后的关系。方法收集来自黄曲霉毒素BI高暴露区TNM分期为l～Ⅱ期的HCC患者（138例）手术切除标本，通过TaqMan--PCR技术检测miR--429在癌组织、癌旁组织、肝癌细胞株SMMC--7721以及非肝癌细胞．株QsG-7701中的表达情况，通过风险比例回归模型及逻辑回归模型分析miR--429表达与HCC临床病理学特征以及预后的关系。结果与癌旁组织相比，miR--429在HCC癌组织中的表达水平上调，升高约4倍（P〈O．01），细胞学实验有类似发现；miR-429表达水平与HCC肿瘤大小及AFBl-DNA加合物水平相关-风险值分别为3．13（1．47～6．67）和2．70（1．28～5．56）；高miR--429表达影响HCC5年期总体生存率及肿瘤无复发生存率（P〈O．01），增加总体死亡风险及肿瘤复发风险分别为3．64倍和5．94倍。结论miR--429表达参与HCC肿瘤化过程，并影响HCC预后。

Abstract： Objective To explore （HCC） and whether miR--429 having microRNA--429 （miR--429） expression in the hepatocellular carcinoma effects on the pathological features and prognosis of HCC. Methods A total of 138 patients with TNM--stage HCC from high exposure to Aflatoxin B1 （AFB1） were recrui-ted and the corresponding surgical resection samples were collected. MiR--429 expression in the tissue samples （including tumor tissues and adjacent tissues of HCC） and cells （ineluding liver cancer cell line SMMC--7721 and non--cancer liver cell line QSG--7701） were tested by using TaqMAN--PCR technique. Cox＇s proportion al hazard regression model and logistic regression model were used to analyze the relationship between miR-- 429 expression and HCC pathological features and prognosis. Results Compared with adjacent tissues, ex- pression level of miR--429 in tumor tissues increased by about 4 times （ P 〈0.01）; similar findings was also observed in the cell analysis. Expression levels of miR--429 were correlated with primary tumor size and AFB1 --DNA adducts level, and the corresponding risk value was 3.13 （1.47-6.67） and 2.70 （1.28-5.56）, re spectively. Elevated level of miR--429 expression is effective on 5--year overallsurvival rate and tumor recur rence--free survival rate （Pal0.01）. The total death risk increased by 3.64 times and tumor recurrence risk increased by 5.94 times. Conclusion These results suggest that miR--429 expression be involved in HCC tumorigenesis and be associated with cancer prognosis.