1-甲基环丙烯对艳红桃采后果实软化和活性氧代谢的影响

Effects of 1-MCP Treatment on Fruit Softening and Active Oxygen Metabolism of ‘Yanhong' Peach

为了给艳红桃果实的采后保鲜贮藏提供参考,以艳红桃果实为材料,采用实验室方法研究1-甲基环丙烯(1-MCP)对其采后贮藏果实软化和活性氧代谢的影响。结果表明:①1-MCP处理的艳红桃果实贮藏到第8天时,其果实的硬度为5.5 kg/cm2,是贮藏前的42.1%;呼吸强度为62.6 mg CO2/(kg·h),但仍处于上升趋势,未达呼吸高峰;可溶性固形物为14.7%,明显低于对照(15.7%),且果实内部物质的转化速度下降;细胞膜透性为29.1%,是贮藏前的1.8倍;果实内的O-·2产生速率为1.34 nmol/(min·g),是对照的82.7%;SOD活性为210.3 U/g,是对照组的1.3倍;CAT活性为9.7 U/g,是对照的1.6倍;POD活性为37.8U/g,是对照的1.3倍。②随着贮藏时间的延长,1-MCP可增加果实贮藏后期的SOD、POD和CAT活性,降低果实中超氧阴离子自由基的积累,抑制细胞膜透性,维持膜结构稳定。因此,1-MCP可有效减缓艳红桃果实贮藏期衰老和软化速度,抑制呼吸强度,推迟呼吸高峰期,减缓可溶性固形物升高的作用。

The effect of 1 -Methylcyclopropene （ 1 -MCP） on fruit softening and active oxygen metabolism of ＇ Yanhong＇ peach was conducted during fruit storage to provide references for post-harvest refreshment. The results showed that： Firstly, on 8th day during storage, fruit firm- ness treated by 1 -MCP was 5.5 kg/cm2, which was 42. 1% of that before storage; the respiratory intensity was 62.6 mg CO2/ （ kg·h）, but still in an upward trend and did not reach to the peak ; the content of soluble solid was 14.7 %, significantly lower than control; relative membrane permeability was 29.1%, 1.8 times higher than before ; the accumulation of superoxlde anion （ O2^- ） was 1.34 nmol/（ min · g）, which was 82.7 % of control; SOD activity was 210.3U/g, higher than control; CAT activity was 9.7 U/g, 1.6 times higher than control ; POD activity was 37.8 U/g, 1.3 times higher than control. Secondly, along with the prolong of storage, SOD, POD, and CAT activity were significantly promoted by 1 -MCP treatment, the accumulation of superoxide anion （ 02＂ ） , the relative membrane permeability was inhibited, and the stability of cell membrane was maintained. Therefore, it could be concluded that 1-MCP could delay the speed of fruit softening and senescence, inhibit respiration intensity, postpone the respiration peak, and inhibit the increase of the soluble solid.