温度、pH对泥鳅消化酶活力影响的研究

Effect of Temperature and pH on Digestive Enzyme Activities of Misgurnus anguillicaudatus

为了研究温度和pH值对泥鳅成鱼肠道和肝胰脏3种消化酶活力的影响。以Folin-酚法、3,5-二硝基水杨酸法及对硝基苯酚法分别测定泥鳅肠道和肝胰脏蛋白酶、淀粉酶及脂肪酶活力。研究发现:泥鳅肠道蛋白酶、淀粉酶和脂肪酶的最适温度分别为40℃、45℃、35℃;肝胰脏蛋白酶、淀粉酶和脂肪酶的最适温度分别为30℃、40℃、35℃。泥鳅肠道及肝胰脏蛋白酶、淀粉酶和脂肪酶的最适pH 7.2。最适温度下,肠道3种消化酶的活力均高于肝胰脏、肠道、肝胰脏A/P值均>1。建议在泥鳅适宜的养殖水温内,应随着水温的升高适当提高泥鳅的投饵量;泥鳅的食性为偏植食性,对糖类具有较好的利用能力,泥鳅的饵料中可适当增加糖类的比重,既可满足泥鳅的营养需求又节约了饲料成本。

The effects of temperature and pH value on digestive enzyme activities of adult Misgurnus- anguillieaudatus were investigated. Folin-phenol, 3,5-dinitrosalicylic acid and p-nitrophenol were prepared to determine the activities of protease, amylase and lipase in M. anguillicaudatus intestine and hepatopanerea. The optimum temperatures of protease, amylase and lipase in intestine were 40℃, 45℃ and 35℃, respectively. The optimum temperatures of protease, amylase and lipase in hepatopancreas were 30℃, 40℃ and 35℃, respectively. All the optimum pH values of three digestive enzymes in intestine or hepatopancreas were 7.2. The A/Pvalues both in intestine and hepatopancreas were greater than 1. The results suggested that the dietary of M. anguillicaudatus could be increased with the rise of water temperature at the appropriate range for M. anguillieaudatus cultivation. M. anguillieaudatus was more like herbivorous, and more capable digestibility for carbohydrate. The formulation of M. anguillieaudatus diet might be more proportion of carbohydrate, it could fulfill the requirement of nutrition and be more commercial.