产广谱抗菌物质芽孢杆菌的筛选与鉴定

Screening and Identification of Antagonistic Bacillus Producing Broad-spectrum Antimicrobial Substances

旨在筛选出对果蔬病原菌具有广谱拮抗作用的芽孢杆菌。以9株病原真菌和3株病原细菌为指示菌,采用琼脂平板扩散法,通过初筛和复筛,筛选得到一株具有广谱抑菌作用的拮抗芽孢杆菌NCPSJ7。结合形态学、生理生化和16S rDNA序列同源性分析等手段,该菌株被鉴定为解淀粉芽孢杆菌(Bacillus Amyloliquefaciens)。抑菌谱实验表明,解淀粉芽孢杆菌NCPSJ7对果蔬病原真菌和病原细菌都具有较强的抑菌能力。该菌株产抗菌物质的适宜发酵培养基、发酵条件优化,抗菌物质的分离纯化、组成、性质和抗菌稳定性等还有待于进一步研究。

The aim was to screen Bacillus with broad antimicrobial spectrum against pathogen of fruits and vegetables. With nine pathogenic fungi and three pathogenic bacteria as indicators, Bacillus NCPSJ7 was screened using agar disk diffusion methods. Combined morphological analysis, physiological and biochemical characteristics, and 16S rDNA gene homology analysis, strain NCPSJ7 was finally identified as Bacillus Amyloliquefaciens. The antimicrobial spectrum of B. Amyloliquefaciens NCPSJ7 was studied. It showed that strain NCPSJ7 displayed both antifungal and antibacterial activity. The optimization of fermentation conditions, the composition, properties and antibacterial stability of the antibacterial substance, and separation and purification of the antibacterial substance will be studied in the further research.