髓核细胞移植抑制椎间盘退变

Nucleus pulposus cell transplantation inhibits intervertebral disk degeneration

背景:近年来,椎间盘细胞移植和椎间盘移植修复椎间盘退变研究取得较大的进展。目的:探讨髓核细胞的体外培养方法以及髓核细胞移植在抑制椎间盘退变研究中的作用。方法:通过数据库检索的方式探讨髓核细胞移植抑制椎间盘退变的研究。髓核细胞的主要功能是产生胶原和蛋白聚糖,改进髓核细胞的培养方法,使培养后的髓核细胞数量增多,合成和分泌细胞外基质增加,通过髓核细胞移植来修复退变的椎间盘。结果与结论:通过三维小球聚集培养、微载体旋转立体培养等方法可以使髓核细胞数量增多,肝细胞生长因子对髓核细胞增殖产生促进作用。髓核细胞移植可以恢复退变椎间盘高度,促进退变椎间盘内蛋白多糖和Ⅱ型胶原的生物合成和分泌。随着对椎间盘退变研究的不断深入,髓核细胞移植对退变椎间盘可能是一种重要的治疗手段。

BACKGROUND： In recent years, cell transplantation and intervertebral disk grafting for intervertebral disk degeneration have been greatly developed.OBJECTIVE： To investigate the in vitro culture methods of nucleus pulposus cells and effects of nucleus puposus cell transplantation on intervertebral disk degeneration.METHODS： A computer-based search of databases was performed to explore the effects of nucleus pulposus cell transplantation on intervertebral disk degeneration. The main function of the nucleus pulposus cells is to produce collagen and proteoglycans. Through improvement of culture methods, the number of nucleus pulposus cells increased, and matrix synthesis and secretion also increased. Therefore, the nucleus pulposus cell transplantation was able to repair the degenerated intervertebral disk.RESULTS AND CONCLUSION： The three-dimensional aggregation culture method and three-dimensional microcarrier rotation method elicit the increase in the number of nucleus pulposus cells, and hepatocyte growth factor also can produce a catalytic role in the proliferation of nucleus pulposus cells. Nucleus pulposus cell transplantation can restore degenerative disc height, and promote the biosynthesis and secretion of proteoglycans and type Ⅱ collagen in the degenerated intervertebral disk. With the deepening of intervertebral disk research, nucleus pulposus cell transplantation may become an important therapeutic tool for intervertebral disk degeneration.