2010-2012年福建省H3N2亚型流感病毒血凝素基因特征分析

Analysis of the hemagglutinin gene characteristics of H3N2 subtype influenza viruses isolated in Fujian,2010-2012

目的通过对2010—2012年福建省分离的H3N2亚型流感毒株血凝素HA1基因进行分析，了解2010-2012年福建省H3N2亚型流感病毒的基因特征和变异规律。方法随机选择28株毒株，提取病毒RNA，采用RT-PCR扩增目的片段，纯化PCR产物后进行测序；利用DNAstar和Mega4．0等生物信患学软件进行核苷酸整理、拼接、校对、差异性比较以及基因进化树构建等分析研究。结果28株H3N2亚型流感病毒HAl区核苷酸同源性在96．4％～100％之间。基因系统进化分析显示，其进化规律都是沿着树枝主干随着时闻推移向，上延伸，序列的差异和年代成正比。2010—2012年间HAl区共出现43个氨基酸位点的变化，其中有10个氨基酸位点变异累计涉及4个抗原决定簇，2个变异位点发生在受体结合位点（RBS）及其附近，应予高度重视。结论相对于A／Perth／16／2009疫苗代表株，2012年的部分H3N2亚型流感出现了抗原漂移；部分毒株已出现氨基酸位点的改变，并涉及抗原决定簇及受体结合位点，提示2010—2012年福建省人群中流行的H3N2亚型流感正逐渐发生变异，应加强流感病原学监测，以及时发现新的流感变异株，为福建省流感防控提供科学依据。

In order to explore the characteristics of hemagglutinin 1 （HA1） gene of H3N2 subtype influenza in Fujian, 28 strains were randomly selected for detection during 2010 to 2012. After the RNA from isolated strains was extracted, frag- ments of HA1 geres were amplified by RT-PCR and sequenced. Our results on genetic evolution indicated that＇ there was a ho＇ mology of 96.4%--100 %：among the HA1 nucleotide sequence of 28 H3N2 subtype influenza ,viruses, ：and the sequence varia- tion rates were proportional to tim6; A total of 43 sites in amino acid vai＇iation was identified in the HA1 region, resulting 10 variations scattered,in four antigen determinants and 7 variations occurred near the receptor binding sites （RBS）. Compared with vaccine strain （A/Perth/16/2009）, there was an antigenic drift in HA1 region in 2012. In conclusion, the H3N2 subtype influenza between 2010 and 2012 from Fujian has a gradually antigenic variation and,more attention should be paid to the sur- veillance of their mutation for control and prevention strategies.