G蛋白耦联的内向整流型钾通道4基因多态性与新疆维吾尔族人群血脂异常的相关性

Relationship between the G Protein Gated Inward Rectifier Potassium Channel 4 Gene Polymorphism and Dyslipidemia of Uyghur Residents

目的研究G蛋白耦联的内向整流型钾通道4(GIRK4)基因多态性与新疆维吾尔族血脂异常的相关性。方法采用TaqmanPCR方法,对GIRK4基因rs2604204、rs4937391、rs6590357、rs11221497多态性在维吾尔族自然人群中进行基因分型,按常规方法测定血脂水平并进行比较分析。结果 50岁以下人群中,三酰甘油异常组和正常组rs6590357位点的基因型分布差异有统计学意义(P=0.005),总胆固醇异常组和正常组rs11221497位点的基因型分布差异有统计学意义(P=0.011)。采用Logistic回归方法校正性别、肥胖等因素后,50岁以下人群中rs6590357位点与TG异常、rs11221497位点与TC异常仍然为阳性关联。rs6590357位点CT+TT基因型组中TG水平明显高于CC组(P=0.014),rs11221497位点CC基因型组中TC水平明显高于CG+GG组(P=0.006)。对其进行单体型分析后发现,H3单体型频率在TG异常组与正常组间差异有统计学意义(P=0.007)。结论 GIRK4基因rs11221497和rs6590357位点多态性可能与新疆维吾尔族血脂异常相关。

Objective To investigate the relationship between the G protein-gated inward rectifier K ＋ channel subunit 4 （GIRK4） gene polymorphism and the dyslipidemia among Uyghur residents in Xin- jiang. Methods The polymorphisms of rs2604204, rs4937391, rs6590357, and rs11221497 among the Uyghur residents were genotyped using Taqman polymerase chain reaction （PCR） . Lipid levels were measured by con- ventional methods and were analyzed. Results In the less-than-50-years population, the genotype distributions of the rs6590357 was statistically significant different in subjects with or without abnormal triglycerides （ P =0. 005 ） . Aslo, the the genotype distributions of the rs11221497 also significantly differed in subjects with nor- mal compared or abnormal TG （ P = 0. 011 ） . Logistic regression analysis suggested that rs6590357 still had pos- itive association with TG abnormalities in subjects under 50 years （P = 0. 014） . rs11221497 also had positive association with TC abnormalities. The TG levels of CT ＋ TY genotypes were significantly higher than the CC group （P = 0. 006） . Haplotype analysis found that the differences of H3 haplotype frequencies between the TG abnormal and normal groups were statistically significant （P = 0. 007） . Conclusion The polymorphisms of rs11221497 and rs6590357 of GIRK4 gene may play a role in the development of dyslipidemia in Uygur population.