用桑蚕丝素蛋白固定的葡萄糖氧化酶传感器

A Sensor of Glucose Oxidase Immobilized in Bombyx Mori Silk Fibroin Membrane

目前,常用的生物酶固定化方法在应用上各有优劣,简便、高效而又适用推广的酶固定化方法是人们所追求的目标。本文提供一种新型的酶固定化方法,即通过蚕丝素蛋白在甲醇中变性,使葡萄糖氧化酶(GOD)固定在蛋白质中,得到葡萄糖氧化酶-丝素膜,与氧电极偶合,制成电流式葡萄糖传感器。在选定的实验条件下,电极的各项性能指标均较满意。 GOD由Boehringer Mannhen GmbH进口(10 kU/46 mg),试剂均为分析纯。家蚕(浙12×苏1)由浙江农业大学提供。氧电极和测氧仪由中国科学院上海冶金研究所制造。

Glucose oxidase (GOD) can be immobilized in Bombyx mori silk fibroin membrane. This is due to the structural transition of the silk fibroin membrane from random coil to antiparalled β-sheet induced by methanol treatment. Glucose sensor was made of this GOD-immobilized fibroin membrane. The calibration curve, response time, recovery, thermal and storage stability were determined.