宿州辣椒地方品种分子遗传多样性分析

Molecular genetic diversity of local pepper varieties in Suzhou

利用优化的辣椒SRAP—PCR反应体系，研究宿州辣椒地方品种的遗传多样性．从30个引物组合中筛选出条带清晰、多态性好的15个引物组合，共扩增出62条谱带．其中，多态性谱带有40条，多态率为64．5％；不同引物扩增的条带为3～7条，谱带大小范围为100～1000bp．供试品种的遗传相似系数为0．52～1．00之间，当遗传距离D=0．52时，可将27份材料分为两大类．结果表明，宿州辣椒种质资源遗传多样性丰富．

The SRAP molecular marker was used to identify the genetic differences of pepper varieties. On the basis of orthogonally optimized SRAP-PCR amplification system, genetic diversity of 27 pepper varie- ties in Suzhou was studied. 15 effective primer combinations were selected from 30 primer-combinations. A total of 62 bands were detected with those primers and 40 of them were polymorphic. The percentage of polymorphy was 64.5 ~. Their Genetic similitary coeffient ranged from 0.52 to 1.00. Most of the am- plified fragments had a length ranged from 100 to 1000 bp. The cluster analysis using DPS software showed that the tested materials could be categorized into 2 groups at D = 0. 52, the cluster results showed that the genetic differentiation of local pepper varieties in Suzhou was abundant.