1,4-二巯基苏糖醇的极谱研究

Studies on Polarographic Behavior of 1,4-Dithiothreitol

1,4-二巯基苏糖醇(简称DTT)是一种重要的生化物质,广泛地应用于蛋白质的分离、测定和生理作用的研究等方面。尽管DTT在生物医学上有重要的作用,但它的分析测定方法报道的却不多,其中电分析方法更为少见。本文研究了它的极谱性质,发现以氯乙酸为介质,在单扫描示波极谱图上DTT产生灵敏的吸附波,此波可用于痕量DTT的测定。 1

In ClCH_2COOH medium, 1,4-dithiothreitol (DTT) can shows a sensitive polarographic wave at-0. 31 V (vs. SCE). In this paper, conditions are found for the determination of 1,4-dithiothreitol with single sweeping polarography. Under the best condition the detection limit is 8×10^(-8) mol/L and the linear range of 1.1×10^(-7)～5×10^(-5) mol/L is exhibited. The polarographic character of DTT is investigated, the polarographic wave resulted from DTT shows the properties of adsorptive catalysis wave. The electrode reaction mechanisms are discussed in the paper and the effect of Cu^(2+)on the polarographic wave is also studied.