RP-HPLC法测定接骨木中盐酸小檗碱的含量研究

Determination of berberine hydrochloride in Sambucus williamsii Hance by RP-HPLC

目的 采用反相高效液相色谱法建立接骨木中盐酸小檗碱含量的测定方法.方法采用反相高效液相色谱法,色谱柱：Hypersil ODS C18（4.6 mm×250 mm,5 μ m）;流动相：甲醇-水-磷酸 （25：75：0.2）;柱温：30 ℃;检测波长：340 nm.结果 盐酸小檗碱在4.90~49.0 μg·mL-1范围内线性关系良好（r=0.999 8）,精密度试验的RSD=0.36%,样品稳定性试验的RSD=1.08%,样品重复性试验的RSD=0.92%,平均回收率为98.68% （RSD=0.55%）.结论反相高效液相色谱法测定接骨木中盐酸小檗碱含量的方法简单、准确度高、重现性好,为制定接骨木的质量控制标准提供参考依据.

Objective To establish a method for the determination of berberine hydrochloride in Sambucus williamsii Hance by RP - HPLC. Methods Using the method of RP - HPLC, the chromatographic column ： Hypersil ODS Cis （ 4.6 mm×250 mm,5 μ m ） ; Mobile phase ： methanol - water - phosphoric acid （ 25 ： 75 ： 0. 2 ） ; Column temperature ： 30 ℃ ; The detection wavelength：340 nm. Results The linear range of berberine hydrochloride was 4. 90 - 49.0μg ~ mL-1 （ r = 0. 999 8） ,the RSD of precision test = 0. 36%, the RSD of sample stability test = 1.08%, the RSD of repeatability test = 0. 92% ,the average recovery was 98.77% （ RSD = 0.77% ）. Conclusion The method for determination of berberirre hydrochloride in Sambucus williamsii Hance was simple, accurate, reproducible, provided reference for the quality control standard of Sambucus williamsii Hance.