有盐条件下微生物对石油的降解效果研究

Study on microorganisms degradation of crude oil under salt-containing conditions

通过摇瓶试验,研究含盐量为7 000 mg·L-1、石油含量分别为10 000 mg·L-1,30 000 mg·L-1,50 000 mg·L-1条件下,空白对照组(不添加微生物)、细菌组、真菌组分别在5,12,19,26,33 d时对石油的降解效果,并对比分析了降解效果.结果表明,经过33 d后,空白对照组中石油含量未变化,细菌组石油含量为10 000 mg·L-1一组的石油降解率最高,达到了65.69%,真菌组石油含量为30 000 mg·L-1一组石油降解率最高,达到了45.84%.可见,在较高的含盐量条件下,细菌和真菌对石油的降解也仍然有显著效果.

In order to study the bioremediation of petroleum pollutions under high salt concentration （7 000 mg ·L-1 ） by bacteria and fungus, the experiment was designed to have three treatments in the culture flask though table test： blank control, bacteria, fungus. Three crude oil contents, including 10 000 mg ·L-1 , 30 000 mg·L-1 and 50 000 mg·L-1. Sample analysis shall be conducted respectively on 5, 12, 19, 26, 33 d. The results show that the crude oil eontent in blank control group didn＇t change greatly; in bacteria group, the degradation rate of petroleum under 10 000 mg·L-1 was 65.69%, in fungus group, 30 000 mg· L -1, it was 45.84%. Therefore, both bacteria and fungus have remarkable effect on the degradation of crude oil.