睾酮类兴奋剂测定中一些β-葡萄糖醛酸甙酶酶解能力的比较

Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control

睾酮是国际奥委会规定禁用的99种兴奋剂之一,由于睾酮是内源性激素,因此不能靠定性来判断运动员是否服用了睾酮,在人体激素代谢中,产生睾酮差向异构体——表睾酮,在男性黄种人中睾酮与表睾酮浓度比约为1:1,白种人中略高一些,根据这一情况,国际奥委会规定,人尿中睾酮与表睾酮之间浓度比不得超过6:1。

Selected Ion Monitor (SIM) of GC-MS is used to determine the amounts of testosterone (T) , epitestosterone(ET) and their ratio in urine.The affection of five β-glucuronidases to the determination of T level has been compared. It is figured out that both the Calf Enzyme (made in China) and the E. Coli(IX-A) from Sigma have strong activities while hydrolyzing the combined T, ET in urine. So they are fit to the determination of T and ET level of urine in doping Control.