手足口病患儿淋巴细胞亚群和免疫球蛋白检测的临床意义

Clinical Significance of Detection of Lymphocyte Subsets and Serum Immunoglobulin in Patients with Hand-foot-mouth Disease

目的研究HFMD外周血淋巴细胞亚群以及血清免疫球蛋白的变化及临床意义。方法采用流式细胞术检测56例HFMD和53例健康对照组外周血CD3+T、CD4+T、CD8+T、CD16+56+NK、CD19+B细胞的百分率;免疫比浊法检测血清IgG、IgA及IgM含量。结果 HFMD与正常对照组比较,CD4+/CD8+比值、CD4+T淋巴细胞百分率及CD19+B淋巴细胞百分率均明显增高(P<0.05);血清IgG水平升高(P<0.001);CD15+56+NK细胞百分率明显降低(P<0.02);CD3+T、CD8+T淋巴细胞的百分率及血清IgM、IgA含量与正常对照组相比差异无统计学意义。结论 HFMD体内细胞和体液免疫功能紊乱,监测HFMD体内淋巴细胞亚群和免疫球蛋白水平,对于HFMD的治疗具有重要的临床意义。

[Abstract] Objective To study the changes of lymphocyte subsets in peripheral blood and serum immunoglobulin of patients with hand-foot-mouth disease （HFMD） and their clinical significance. Methods CD3 ＋T, CD4 ＋T, CD8 ＋T, CD16 ＋56 ＋NK, CD19 ＋B positive rate （with flow cytometry） and serum IgG, IgA, IgM levels （with immunoturbidimetry ） were measured in 56 patients with HFMD and 53 normal controls. Results Compared with the normal controls, the CD4 ＋/CD8 ＋ratio, percentage of CD4 ＋T lymphocyte, and CD19＋B cell percentage increased significantly （P〈0.05, P〈0.005, P〈0.02） in HFMD patients; IgG level slightly increased （P〈0.001）, while CD15＋56＋NK cell percentage reduced obviously （P〈0.02）. There were no statistical differences between HFMD patients and normal controls about the levels of CD3 ＋T, CD8 ＋T cell and IgM, IgA. Conclusion Cellular and humoral immune function were significantly abnormal in patients with HFMD. Detection of lymphocyte subsets and serum immunoglobulin had important clinical significance for HFMD＇s immunotherapy.