植株内水杨酸诱导表达与花生网斑病抗性的关系

Endogenous salicylic acid expression and its resistance to peanut web blotch

为探讨水杨酸(SA)在花生网斑病诱导抗性中的作用,采用ELISA方法测定花生植株不同生长期、不同位置叶片及不同组织中内源SA含量,然后将2%的网斑病菌培养滤液喷施于花生叶片上,研究诱导接种后叶片中SA含量变化与诱导抗性的关系。结果表明:未诱导接种花生生长中期叶片及根组织中SA含量相对稳定,各品种间差异不显著,但叶片中SA含量显著高于根中含量;诱导处理叶片和临近非处理叶片SA含量变化曲线都有两个高峰值,第一个高峰值出现时间和峰值大小叶片间差异不明显,而第二个高峰值出现时间及大小和叶片位置关系密切。初步确定SA在花生网斑病抗性及信号转导中发挥着重要作用。

To identify the role of salicylic acid （SA） in peanut induced resistance to peanut web blotch, the content of endogenous SA in leaves and roots of peanut plants were measured by Enzyme - Linked Immunosorbent Assay （ELISA） method. Then the changs of SA content in leaves at different location inoculated with 2% cultural filtrate of Phoma arachidicola were monitored, and their relationship with induced resistance were analyzed. The results indicated no significant differences in content of SA in leaves at different locations without inoculation at the same growth stage. However, the content of SA in leaf was higher than in roots. After treated with 2% culture fil- trate, the content of SA reached its peak twice both in treated leaves and untreated nearby leaves. At first peak, there were no significant differences in both peak time and peak values between different leaves. At second peak, both peak time and peak values were closely related to leaves in different locations. It was concluded that SA played a significant role in peanut web blotch resistance and signal transduction.