荧光各向异性法研究环糊精包络物

Studies on Cyclodextrin Inclusion Complex with Fluorescence Anisotropy

推导了用荧光各向异性法测定环糊精包络物平衡结合常数的方程式,并用于测定芘-β-环糊精包络物的结合常数,获得了满意的结果,依据推得的方程式并结合各向异性～环糊精浓度曲线讨论了环糊精包络物存在形式及主-客体作用方式,预计了以蔡衍生物为配体的金属离子分析方法应用β-环糊精的可能性。

An equation for the determination of equilibrium association constant (KA) of cyclodextrin inclusion complex with fluorescence anisotropy was derived and used to determine KA of pyrene-β-cy-clodextrin inclusion complex satisfactorily. The existing form of inclusion complex and the host-guest interaction were discussed based on the derived equation along with the curve of fluorescence anisotropy versus cyclodextrin concentration for guest/cyclodextrin system. The possibility of application β- cyclodextrin in the analysis of metal ion using naphthalene derivative as a ligand was predicted.