青刺果分离提取物的抑菌活性研究

On Anti-bacteria Activity of Separation Extracts from Prinsepia Utilis Royle Fruit Meal in Vitro

目的:探讨青刺果分离提取物对金黄色葡萄球菌、大肠杆菌、沙门氏菌3种常见致病细菌的体外抑制作用.方法:应用滤纸片扩散法和微量肉汤稀释法(micro broth dilution)分别检测青刺果分离提取物对试验菌的抑菌圈直径、最低抑菌浓度(Minimum Inhibitory Consistency)以及最低杀菌浓度(Minimum Bactericidal Consistency).结果:青刺果分离提取物经AB-8大孔树脂洗脱后抑菌活性物质富集在30%醇洗部分,对三种标准菌(金葡球菌ATCC25923、大肠杆菌44102、沙门氏菌50041)的抑菌圈直径分别为20.90 mm、19.81 mm、19.53 mm),三种耐药菌(金葡球菌GL17、大肠杆菌EYAC08-56、沙门氏菌STQD2G)的抑菌圈直径分别为17.89mm、19.61mm、17.42 mm,同时,MIC值结果表明大孔树脂洗脱的30%醇洗部分对标准的金葡球菌ATCC25923和大肠杆菌44102的MIC值(12.5 mg/mL、25 mg/mL)是洗脱前对标准的金葡球菌和大肠杆菌MIC值(25 mg/mL、50 mg/mL)的二分之一,对标准沙门氏菌50041的MIC值(25 mg/mL)仅为洗脱前分离提取物对标准沙门氏菌MIC值(100 mg/mL)的四分之一.结论:青刺果分离提取物对标准和耐药的金黄色葡萄球菌、大肠杆菌、沙门氏菌等6种菌株均有不同程度的抑菌或杀菌能力.

Objective： To study the antimicrobial activity of Separation extracts from Prinsepia Utilis Royle Fruit Meal against Staphylococcus aureus, Escherichia coli and SaLmonella sp. Methods： The antibacterial circle diameter was tested by the method of Paper- dispersion, and the application of micro broth dilution （BMD） de- tected Minimum inhibitory consistency （MIC） and Minimum bactericidal consistency （MBC）. Results： Antibac- terial substances of the Separation extracts from Prinsepia Utilis Royle Fruit Meal were enriched in 30% alcohol ex- tract by AB -8 macroporous resin elution. Standard Strains （Staphylococcus aureus ATCC25923, Escherichia co- li44102 and SaLmonella sp50041 ） of antibacterial circle diameter was 20.90 ram, 19.81 mm and 19.53 mm, as for drug- resistant strains, （Staphylococcus aureu GLI7, Escherichia coli EYAC08 -56 and SaLmonella sp, STQD2G ）, its antibacterial circle diameter was 17.89mm, 19.61mm and 17.42 mm, respectively. The MIC values of the 30% alcohol extract （ 12.5mg/ml, 25 mg/ml） against Staphylococcus. aureus ATCC25923 and Esche- richia coli44102 were half of Crude extract, SaLmonella sp50041 （25 mg/mL） was one -quarter of Crude extract （ 100 mg/mL）. Conclusion： The extracts from Prinsepia Utilis Royle Fruit Meal had different levels of antibacteri- al or bactericidal activity against standards and resistant of Staphylococcus aureus, Escherichia coli and SaLmonella sp in vitro.