Wnt3a对非小细胞肺癌细胞生长的调控作用

The regulation of Wnt3a on the cell growth in non-small-cell lung cancer cell lines

目的:观察重组人Wnt3a对非小细胞肺癌细胞生长的调节作用,为进一步探讨Wnt信号通路在非小细胞肺癌的作用提供实验依据。方法:本实验利用重组人Wnt3a处理H460、A549细胞系,通过自动细胞计数、软琼脂克隆形成实验、流式细胞周期分析,观察重组人Wnt3a处理对细胞生长和细胞周期的作用。结果:Wnt3a处理A549、H460细胞24h后,自动细胞计数结果显示细胞数上升显著[A549:(4.26±0.26)×105/ml vs(2.42±0.44)×105/ml,P<0.01;H460:(3.98±0.21)×105/ml vs(2.07±0.10)×1 05/ml,P<0.0 1];软琼脂克隆实验显示克隆形成效率显著提高[A549:(1.57±0.11)%vs(1.03±0.08)%,P<0.01;H460:(1.53±0.11)%vs(0.79±0.10)%,P<0.01];流式细胞仪细胞周期分析显示,Wnt3a处理组G0/G1期细胞比例下降,S期细胞比例上升。结论:在A549和H460细胞,重组人Wnt3a处理,可能调节细胞周期,促进细胞生长。

Objective：To approach the molecular mechanism of Wnt signaling pathway in non - small - cell lung cancer（NSCLC） ,and the effect of recombinant human Wnt3a on NSCLC cell lines. Methods：H460 and A549 cell lines were treated with recombinant human Wnt3a. The alteration of the cell numbers, colony forming in soft -agar and cell cycle were observed. Results ：The treatment of recombinant human Wnt3 a significantly increased cell numbers [ （4.26 ±0. 26） ×10^5/ml vs （2.42 ±0.44）×10^5/ml in A549,P 〈 0. 01 ; （3.98 ±0. 21 ）×10^5/ml vs （ 2.07 ±0.10 ） ×10^5/ml in H460,P 〈 0.01 ] and the efficiency of colony forming[ （ 1.57 ±0.11 ） % vs （ 1.03 ±0.08 ） % in A549, P 〈 0.01 ; （ 1.53 ±0.11 ） % vs （0.79 ±0.10） % in H460, P 〈 0.01 ]. The flow eytometric analysis showed that the cells of G0/G1 phase declined and the cells of S phase rose after the treatment of recombinant human Wnt3a. Conclusion ：As an activator of canonical Wnt signaling,recombinant human Wnt3a may promote cell growth through its regu- lation to cell cycle in NSCLC cell lines.