自体表皮细胞-纤维蛋白膜创面移植治疗大鼠烧伤

Treatment of Burn by Transplanting Fibrinous Membrane with Autologous Epidermal Cells on Raw Surface

目的:观察自体表皮细胞-纤维蛋白膜移植到大鼠烧伤创面治疗皮肤缺损的效果。方法:健康Wistar大鼠20只,随机分成烧伤皮肤缺损造模组和自体表皮细胞-纤维蛋白膜移植治疗组,治疗后计算表皮细胞在纤维蛋白膜上最佳接种密度,观察移植后的各组创面愈合情况、创面伤口的收缩比例等。结果:在纤维蛋白膜上接种表皮细胞的最佳密度为5×104/㎝2,烧伤皮肤缺损造模组创面完全愈合时间平均22.3 d,自体表皮细胞-纤维蛋白膜移植治疗组为18.1 d,造模组创面收缩率为(70±5)%,移植组为(20±5)%(均P<0.05)。结论:自体表皮细胞-纤维蛋白膜可用于覆盖大面积烧伤造成的皮肤缺损,预防创面伤口瘢痕化的形成,减轻创面收缩率,加速皮肤缺损创面的愈合速度。

Objective To observe the treatment effect of skin defect by transplanting fibrinous membrane with autologous epidermal cells to burn wounds in rats, and to look for new ways to treat the burn caused by large area skin defect. Methods Twenty healthy Wistar rats were randomly divided into burn skin defect mod- ule group and fibrinous membrane with autologous epidermal cells transplanting group. The optimum epidermal cells inoculation density on fibrinous membrane was calculated. The wound healing and the wound contraction ratio after fibrinous membrane with autologous epidermal cells transplantation were observed. Results The best density on fibrinous membranes with epidermal cells was 2.5×10^4/cm2, burn skin defect module group rats wound healed completely spening an average of about 22.3 days, and the fibrinous membrane with autologous epidermal ceils transplantation treatment group rats wound healed completely time was about 18.1 days on aver- age; the wound shrinkage rate of burn skin defect module group rats was（70±5）% , and transplantation group rats was （20±5）%. Conclusion The fibrinous membrane with autologous epidermal cells can be used to cover large area skin defect caused by burn, and can prevent scar formation, reduce wound shrinkage rate, to acceler- ate wound healing, thus playing an important role in curing large burn skin defect.