血脉通胶囊对大鼠急性心肌梗死的保护作用及其机制研究

Protective effects of Xuemaitong capsule on rats with acute myocardial infarction and its mechanism

目的:研究血脉通胶囊对大鼠急性心肌梗死(acutemyocardial infarction,AMI)的保护作用并对其作用机制进行初步探讨。方法:试验大鼠随机分为:模型组、假手术组、血脉通胶囊低、中、高剂量组。血脉通胶囊各给药组连续灌胃给药14 d后,除假手术组外,其余各组均结扎大鼠冠状动脉建立AMI模型。记录结扎前及结扎后15、30、60、90、120 min、24 h心电图变化;HE染色和硝基四氮唑蓝染色观察心肌损伤程度和梗死范围的变化;RT-PCR检测基质金属蛋白酶-9(matrix metalloproteinase,MMP-9)、核因子-κB(nuclear factor-κB,NF-κB)mRNA的表达;免疫组化检测MMP-9、NF-κB蛋白的表达。结果:和模型组比较,血脉通胶囊各剂量组可不同程度对抗AMI心电图J点的上抬;减少心肌梗死范围和损伤程度;其中高、中剂量组可减少心肌MMP-9、NF-κB mRNA及蛋白的表达(P<0.05或P<0.01)。结论:血脉通胶囊对结扎大鼠冠状动脉致AMI有明显的保护作用,降低心肌组织MMP-9、NF-κB mRNA及蛋白的表达可能是其作用机制之一。

Objectlve：To investigate the protective effects of Xuemaitong capsule on experimental rats with acute myocardial infarction （AMI） and to explore its mechanism. Methods ： Wistar rats were randomized into 5 groups ： model group, sham operation group, three different dose groups of Xuemaitong capsule preconditioning. Rats in model group were treated by continuous intragastric administra- tion for 14 d. After 14 d,myocardial infarction of all groups was established by ligation of coronary artery except sham operation group.Electrocardiogram was recorded before ligation and at 15,30,60,90,120 min and 24 h after ligation. Pathological changes and infarcted area of cardiac tissues were checked by HE staining combined with nitroblue tetrazolium staining.Immunohistochemical staining, RT-PCR and image analysis were used to measure expressions of matrix metalloproteinase-9 （MMP-9） and nuclear factor-κB （NF- κB） in the myocardial infarction. Results：J points in electrocardiogram in Xuemaitong capsule treated groups were elevated at differ- ent degrees compared with that in model group. Meanwhile, pathological aheration of myocardial infarction in Xuemaitong capsule treated groups was ameliorated compared with that in model group. Immunohistochemical staining indicated that protein and mRNA expressions of MMP-9 and NF-κB were decreased in high dose group and middle dose group（P〈0.05 or P〈0.01 ）. Conclusions ：Xue- maitong capsule has protective effects on AMI of rats induced by ligation of coronary artery, decreased protein and mRNA expressions of MMP-9 and NF-κB may be one of the mechanisms.