胃癌淋巴管上皮β-catenin表达与癌转移相关性的研究

ESPRESSION OF β- CATENIN IN GASTRIC CARCINOMA LYMPHATIC VESSEL AND ITS ASSOCIATION WITH CANCER METASTASIS

目的:通过试验来研究胃癌组织微淋巴管β-catenin的表达及癌细胞转移之间的关系与发生机制。方法:正常胃组织标本2例,胃癌病理组织32例。采用免疫组化方法,通过LYVE-1标记定位,对比不同条件下的转移与分化间的胃癌淋巴管β-catenin的表达情况。结果:LYVE-1在正常组织及癌组织中的微淋巴管上皮呈表达阳性,而正常组织微淋巴管β-catenin表达呈阴性,胃癌组织β-catenin在胃癌微淋巴管中表达不显著,β-catenin在微淋巴管的表达低分化表达率为66.4%,在有转移组中表达率67.7%,分别同高分化组和无转移组表达差异显著(P<0.05)。结论:癌细胞的转移与胞质中β-catenin积累相关,促进了胃癌细胞周围微管道形成,提高了癌细胞的转移几率。

Objective：This the experiment is aimed at researching the relationship between the lymphatics β -catenin expression and metastasis of cancer cells and the mechanism. Methods：2 cases of normal gastric tissue specimens, 32 cases of gastric cancer tis- sue were adopted. Using immunohistochemical method, the expression of LYVE - 1 marker location, lymph node metastasis and differ- entiation between different Conditions of the pipe ,the beta- catenin. Results：The LYVE- 1 in normal tissue and carcinoma lymphatic epithelial was positive expression in normal tissues, and the expression of β- catenin lymph node negative, gastric cancer tissue β- catenin in gastric cancer lymphatic expression is not significant, β-catenin in low differentiation rate was 66.4% the expression of lymphatic vessels, in a rate of 67. 7% expression of metastasis, were different in well differentiated carcinoma metastasis and no expres- sion was significantly （ P 〈 0. 05 ）. Conclusion：The metastasis of cancer cells and beta cytoplasmic - catenin accumulation are directly related, cytoplasmic directly contributes to gastric cancer cells the micro tube formation and around promotes the metastasis of cancer cells.