CDC25C蛋白和CLDN6蛋白在肝细胞癌中的表达及临床意义

Expression of CDC25C and CLDN6 proteins in hepatic cell carcinoma and its clinical significance

目的:研究肝细胞癌(hepatic cell carcinoma,HCC)组织中细胞分裂周期蛋白25同源蛋白C(cell division cyclin 25 homolog C,CDC25C)与紧密连接蛋白Claudin-6(CLDN6)的表达情况,探讨其与HCC的发生、发展、预后的关系。方法:应用免疫组织化学SABC法检测58例HCC、24例癌旁组织和18例正常肝脏组织中CDC25C和CLDN6的表达情况,并分析两者与临床病理特征和术后无瘤生存期的关系。结果:CDC25C在HCC组织、癌旁组织、正常肝脏组织表达阳性率分别为86.2%、45.8%、11.1%,表达差异均有统计学意义(P<0.05)。CDC25C在HCC组织中的表达与乙型肝炎表面抗原(HBsAg)、肿瘤直径大小和肿瘤分化程度有关(P<0.05)。CLDN6在HCC组织、癌旁组织、正常肝脏组织表达阳性率分别为77.6%、41.7%、16.7%,表达差异均有统计学意义(P<0.05)。CLDN6在HCC组织中的表达与肿瘤直径大小有关(P<0.05)。HCC中CDC25C阳性组及CDC25C阴性组的中位无瘤生存时间分别为12个月和15个月,差异有统计学意义(P<0.05)。CLDN6阳性组及CLDN6阴性组中位无瘤生存时间分别为12个月和14个月,差异有统计学意义(P<0.05)。结论:CDC25C和CLDN6可能在肝细胞癌的发生、发展及复发过程中起重要作用,有望成为肝癌诊断及预测预后的重要指标。

Objective： To study the expression of cell division cyclin 25 homolog C （CDC25C） and Claudin-6（CLDN6） proteins in hepatic cell carcinoma（HCC） and discuss its clinical significance with the occurrence, development and prognosis of HCC. Methods： Immunohistochemical SABC method was performed to detect the expression of CDC25C and CLDN6 proteins in 58 cases of HCC tissues, 24 cases of tumor-adjacent tissues and 18 cases of normal liver tissues. The relationship between clinical pathological characteristics and postoperative disease-free survival time was ana- lyzed. Results： The expression positive rates of CDC25C in HCC tissues, tumor-adjacent tissues and normal liver tissues were 86.2% , 45.8% , 11.1%. The differences were statistically signifi- eant（P 〈0.05）. The expression of CDC25C in HCC tissues was closely related to HBsAg, tumor size and tumor differentiation degree（P 〈 0.05）. The positive expression rates of CLDN6 in HCC tissues, tumor-adjacent tissues and normal liver tissues were 77.6% , 41.7% , 16.7% , the differ- ences were statistically significant （P 〈 0.05 ）. The expression of CLDN6 in HCC tissues was closely related to tumor differentiation degree （P 〈 0.05 ）. The median disease-free survival time of CDC25C positive and negative groups was 12 months and 15 months respectively, the differences were statistically significant（P 〈 0.05）. The median disease-free survival time of CLDN6 positive and negative groups were 12 months and 14 months respectively. The differences were statistically significant（P 〈 0.05）. Conclusion： CDC25C and CLDN6 may play important roles in the proces- ses of the occurrence, development and recurrence of HCC. They are expected to be the important indicators for diagnosis and prognosis of HCC.