不同比例自体骨与羟基磷灰石混合物在裸鼠皮下异位成骨的实验研究

The experimental study of subcutaneous ectopic ossification with different proportional mixtures of human autologous bone and hydroxyapatite in nude mice

目的:观察羟基磷灰石(hydroxyapatite,HAP)与人下颌骨不同比例的混合物在裸鼠皮下异位成骨的效果。方法:取人下颌骨外斜线自体骨后,与HAP按一定比例混合,分为5组,A组为自体骨/HAP=2/1,B组为自体骨/HAP=1/1,C组为自体骨/HAP=1/2,D组为自体骨/HAP=1/4,E组为单纯HAP。5组混合物分别植入6只8周龄的裸鼠皮下。植入8周后切取标本,分别行硬组织切片,苦味酸染色后统计新骨形成情况,采用SPSS 13.0软件包对数据进行统计学分析。结果:硬组织切片结果显示,异位新骨形成面积分别为A组9.1%,B组16.1%,C组6.1%,D组3.8%,E组1.3%。B组新骨形成面积最多,E组最少。B组与其他4组相比,各组之间具有显著差异(P﹤0.05)。但C组与D组和E组相比,新骨形成无显著差异(P﹥0.05)。HAP残留率与新骨形成面积呈反比,E组最多(30.3%),而A组材料残留率最小(16.3%)。结论:8周内,裸鼠皮下人自体骨与HAP混合后有助于HAP新骨的形成,最佳比例为1:1,可作为临床上牙种植时上颌窦外提升植人工骨的参考。

PURPOSE： To observe the effect of subcutaneous ectopic osteogenesis using different proportional mixtures of hydroxyapatite （HAP） and human mandible in nude mice. METHODS： After obtaining external oblique ridge of mandible, autologous bone was mixed with HAP according to certain proportion. The mixtures were divided into 5 groups （group A： autologous bone/HAP=2/1; Group B： autologous bone/HAP=l/l; Group C： autologous bone/HAP=l/2; Group D： autologous bone/HAP=a quarter; Group E： HAP）. The 5 groups of mixtures were respectively implanted into nude mice subcutaneously. After 8 weeks, the specimens were obtained and hard tissue sections were completed. The new bone formation was measured after trinitrophenol staining. The data was analyzed with SPSS 13.0 software package. RESULTS： The hard tissue section showed that new ectopic bone formation area was 9.1% （Group A）, 16.1% （Group B）, 6.1% （Group C）, 3.8% （Group D）, and 1.3% （Group E）, respectively. New bone formation was the most in group B, while it was the least in group E. Significant differences were found between group B and other groups （P〈0.05）. However, there was no significant difference between group E and group C or D （P〉0.05）. The retention rate of HAP was the most in group E （30.3%） and the least （16.3%） in group A. CONCLUSIONS： During 8 weeks, human autologous bone mixed with HAP helps new bone formation in nude mice. The best ratio autologous bone and HAP was 1：1. This study provides certain reference basis for dentist to make lifting surgery outside the maxillary sinus using artificial bone graft. Supported by Science and Technology Project of Ningxia Hui Autonomous Region （201 lzys271）.