尾吊、噪声及两因素复合对小鼠免疫功能的影响及柘木多糖的防护作用

Effects of Tail-suspension and( or) Noise Exposure on Immune Function in Mice and the Protection of Cudrapolysaccharide

目的研究尾吊、噪声及两因素复合对小鼠免疫功能的影响及柘木多糖的保护作用。方法利用MTS比色法测定淋巴细胞增殖,ELISA法测定细胞因子,观察7 d尾吊、2 h噪声及两因素叠加处理对小鼠脾T淋巴细胞增殖、细胞因子产生以及腹腔巨噬细胞细胞因子产生的影响。结果 2 h噪声能提高Th1细胞免疫功能,但抑制巨噬细胞功能,7 d尾吊对Th1、Th2细胞免疫功能具有抑制作用,但能刺激巨噬细胞功能;柘木多糖对尾吊、噪声叠加应激引起的T淋巴细胞增殖下降、IL-2分泌减少、巨噬细胞TNF-α产生下降有一定的提升作用。结论急慢性应激因素对免疫细胞的影响具有细胞特异性;柘木多糖对尾吊和噪声这两种急慢性应激引起的免疫功能下降有一定的防护作用。

Objective To investigate effects of tail-suspension, noise exposure and combination of both on the immune function in mice, and study the protection of cudrapolysaccharide （CPS） against immune changes. Methods Lymphocyte proliferation and the cytokine were respectively measured with MTS eolorimetric method and ELISA. Effects of tail suspended for 7 days, 2 h noise exposure and conbimation of both on the splenic lymphocyte proliferation, the cytokine production of splenic cells and peritoneal macrophages were observed and analyzed. Results Noise exposure for 2 h could increase immune function of Thl cells, but decrease mac- rophage function. Seven days tail-suspension could inhibit the immune functions of both Thl and Th2 cells, but would stimulate macrophage function. CPS could inverse the effects of the combination of tail-suspension and noise exposure on T lymphocyte proliferation, IL-2 secretion of splenic lymphocytes and TNF-αproduction of maerophages. Conclusion The effects of acute and chronic stresses on immune cells show cell specificity. CPS can resist immunosuppression induced by combination of tail-suspension and noise exposure.