水稻基因组DNA提取方法的研究

Study on Extraction Method of Rice Genomic DNA

为探索分子标记辅助选择育种中快速有效的DNA提取方法,以水稻新鲜叶片、衰老叶片、水稻种子为材料,以CTAB法和SDS法为提取方法,利用紫外分光光度计和琼脂糖凝胶电泳进行DNA纯度检测,对水稻基因组DNA提取方法和实验体系进行了优化和摸索。结果表明:新鲜叶片提取效果最好,其次是种子,再次是衰老叶片;CTAB法和SDS法差别不大;为避免酚类物质氧化污染,可在提取液中添加β-巯基乙醇。

In order to study fast and effective extraction method of rice genomic DNA for molecular marker-as-sisted breeding ,taking rice fresh leaves ,senescing leaves and rice seeds as experimental materials and CTAB and SDS as methods ,DNA purity was tested by UV spectrophotometer and agarose gel electrophoresis ,extrac-tion methods and experimental system of rice genomic DNA were optimized and explored .The results showed that quality extracted from fresh leaves was the best followed by seeds and senescing leaves was the last ;the difference between CTAB and SDS methods was not very obvious ,andβ-mercaptoethanol could added into so-lution to avoide the pollution of phenols oxidation .