直肠癌新辅助化疗前后血清VEGF与VEGFR-2的变化及临床意义

Clinical significance of the serum levels of VEGF and VEGFR-2 in patients with rectal cancer before and after neoadjuvant chemotherapy

目的探讨直肠癌患者新辅助化疗前后血清血管内皮生长因子(VEGF)及血管内皮细胞生长因子受体2(VEGFR-2)水平的变化及临床意义。方法应用ELISA法检测45例直肠癌患者(化疗组)新辅助化疗前后血清VEGF与VEGFR-2的表达水平,并与45例正常健康人(对照组)作比较。结果化疗组新辅助化疗前血清VEGF、VEGFR-2水平均明显高于对照组(P均<0.01)。新辅助化疗8周后,完全缓解及部分缓解组VEGF、VEGFR-2表达水平均较化疗前明显降低(P均<0.05),降低程度明显高于稳定组(P均<0.01);稳定组化疗后VEGFR、VEGF-2较化疗前显著降低(P均<0.05);进展组化疗后VEGF、VEGFR-2水平均较化疗前显著升高(P均<0.05)。化疗前VEGFR水平与VEGF-2表达呈正相关关系(P<0.01)。结论直肠癌患者血清中VEGFR、VEGF-2均高表达。测定直肠癌患者新辅助化疗前后血清VEGFR、VEGF-2水平的变化有助于临床判断化疗效果及肿瘤进程。

Objective It is to approach the clinical significance of the serum level changes of vascular endothelial growth factor （VEGF） and vascular endothelial growth factor receptor - 2 （ VEGFR - 2） in patients with rectal cancer before and after neoadjuvant chemotherapy. Methods The serum levels of VEGF and VEGFR - 2 of 45 cases with colon carcinoma patients were detected by ELISA and which were compared with those in 45 healthy people. Results The serum levels of VEGF and VEGFR- 2 in colon carcinoma patients before neoadjuvant chemotherapy were significantly higher than those in the healthy people （P 〈0.01 ）. The serum levels in complete remission and partial remission patients in eight-weeks post-chemotherapy were significantly lower than those in pre-chemotherapy （ P 〈 0.05 ） , and lower levels were obviously higher than the stable group （ P 〈 0.01 ）. The VEGF, VEGFR - 2 levels in the stable treatment patients were also lower than that of before neoadju- vant chemotherapy （ P 〈 0.05 ）. The serum levels in the progression group were markedly higher than those of before treatment （ P 〈 0.05 ）. There was positive correlation in serum levels of VEGF and VEGFR - 2 before chemotherapy （ P 〈 0. 01 ）. Con- clusion The serum levels of VEGF and VEGFR - 2 in rectal cancer are high expression. The determination of VEGFR and VEGFR - 2 levels changes in patients with rectal cancer before and after neoadjuvant chemotherapy is helpful to determine the effecl of chemotherapy and the progression of tumor.