聚乙烯醇高含水胶固定化脲酶的研究

Studies on the Behavior of Urease Immobilized by Cross-Linked Polyvinyl Alcohol Gel

冷冻-部分脱水法制成的聚乙烯醇高含水胶固定化脲酶活力收率及对脲素的分解能力明显高于聚丙烯酰胺囱定化脲酶,稳定性相近;用低浓度的戊二醛后处理,提高了固定化酶的稳定性。

The present paper reports the immobilization of urease on 'cross-linked' PVA gel containing a high amount of water. Experimented results show that the immobilized urease on PVA gel has a high activity yield(79%). The immobilized urease activity is 63 mg/g (half hour at 37℃). The repeated determination with substrate showed no detectable loss in enzymatic activity. The determination of the activity within 26 days at 37℃showed a 26% loss of enzymatic activity.