氧化白藜芦醇在大鼠体内的药动学及LC-MS测定法研究

LC-MS/MS method for the determination of oxyresveratrol in rat plasma and its application to pharmacokinetic study

目的:为考察氧化白藜芦醇在大鼠体内的药动学过程,建立液质联用测定方法。方法:采用液液萃取法对大鼠血浆样品进行预处理,建立以卡马西平为内标,色谱柱为Alltima HPLC C18column(100 mm×2.1 mm,3μm),流速为0.4 mL·min-1的LC-MS/MS检测方法。结果:该方法可以用于大鼠给药氧化白藜芦醇后的血药浓度。结论:该方法耗时短(每个样品仅用3 min),回收率高,专属性强,且准确度和精密度均可满足体内药物分析的要求。

Objective： To develop and validate a sensitive and selective liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method for determination of oxyresveratrol in rat plasma. Methods： The ana- lyre and internal standard （carbamazepine） were extracted from plasma samples using simple liquid-liquid extrac- tion technique with ethyl acetate and chromatographed on Alhima HPLC Cls column （100 mmx 2. 1 ram, 3 p.m） -1 coupled with guard column using acetonitrile-0.2% formic acid as mobile phase at a flow rate of 0.4 mL. rain The detection was carried out in multiple reaction monitoring （MRM） mode via positive electrospray ionization （ESI） interface. Results： The method was validated in terms of linearity, specificity, matrix effects, sensitivity, recovery, and accuracy. Conclusion： The method can be used for determination of oxyresveratrol in rat plasma.