摘要
目的 测定平糖胶囊C方(浓缩型)中阿魏酸和淫羊藿苷的含量.方法 采用高效液相色谱法测定,阿魏酸的色谱条件为:色谱柱:XDB-C18(4.6×250mm,5μm),流动相:甲醇-0.5%醋酸水(25:75),流速:1mL·min-1,检测波长:320nm,检测温度:25℃;淫羊藿苷的色谱条件为:色谱柱为XDB-C18(4.6×250mm,5μm)流动相:乙腈-水(23.5:76.5),流速:1mL·min-1,检测波长:270nm,检测温度:25℃.结果 阿魏酸和淫羊藿苷在各自色谱条件下均得到很好的分离,分别在2.6~52μg·mL-1和40~200μg·mL-1范围内呈良好的线性关系,平均加样回收率分别为102.896%和102.4044%.结论 该方法简便、准确、可靠,可用于该制剂的质量控制.
OBJECTIVE To determinate the ferulic acid and icariin in C prescription of PingTang capasules. METHODS Chromatographic separation of ferulic acid was performed on a XDB-C18column (4. 6 × 250mm,5μm) with methanol-0. 5% acetic acid water (25:75) at 25℃ ,the flow rate was 1.0mL·min-1 and the analytes were detected at 320nm; Chromatographic condition of icariin was the similar with ferulic acid, in addition to mobile phase was acetonitrile-water (23.5 : 76. 5 ) and detection wavelength was 270nm. RESULTS The linear range of ferulic acid and icariin were respectively 2. 6 - 52μg·mL^-1 and 40 - 200μg·mL^-1 ,and the average recovery of adding sample were 102. 896% and 102. 4044%. CONCLUSION This method is simple,quick,accurate and reliable, which can be used for the quality control of the preparation.
出处
《海峡药学》
2013年第12期74-76,共3页
Strait Pharmaceutical Journal
基金
国家中医临床研究基地"龙医团队
龙医学者"项目(项目编号LYTD-14)资助
关键词
平糖胶囊C方
阿魏酸
淫羊藿
含量测定
Cpreseription of PingTang eapasules
Ferulie acid
Ieariin
Contnet determination