摘要
目的 测定左乙拉西坦片含量方法的确定.方法 采用HPLC法,色谱柱：C18柱（250mm×4.6mm,5μm）,流动相：庚烷磺酸钠溶液（pH值为2.8）-乙腈（23：2）,流速为1.0mL·min-1,检测波长：220nm,柱温：25℃.结果 左乙拉西坦片的质量浓度在0.1mg·mL-1～0.7mg·mL-1范围内与峰面积呈良好线性关系,回收率均在98%～102%范围内,RSD为0.39%,在8h内供试品溶液质量稳定.结论 该检测方法准确可行,可采用其对左乙拉西坦片进行含量测定.
OBJECTIVE To establish determination method for Levetiracetam Tablets. METHODS The determination was performed by HPLC using C 18 （250mm × 4.6mm, 5 μm）. The mobile phase was heptane sulfonic acid sodium solution （ pH 2.8） -acetonitrile （23： 2）. The flow rate was at 1.0mL · min - 1. The detection wavelength was 322nm and the column temperature was 25℃. RESULTS The calibration cure of Levetiracetam Tablets was Performed over the range of 0.1mg· mL-1 - 0. 7mg · mL-1. The average recovery was 98% - 102% ,RSD was 0. 39% The quality of Levetivacetan was stable in 8h. CONCLUSION The method is accurate and reliable to determin the content of Levetiracetam tablets.
出处
《海峡药学》
2013年第12期84-86,共3页
Strait Pharmaceutical Journal
