HPLC法测定奶粉中蔗果三糖、蔗果四糖和蔗果五糖的含量

Determination of 1-Kestose、Nystose and 1F-Fructofuranosylnystose in milk powder by HPLC

目的 采用HPLC法建立同时测定奶粉中蔗果三糖、蔗果四糖和蔗果五糖的方法.方法 色谱柱Aglient NH2柱（250mm×4.6mm,5μm）,流动相为乙腈-水（70：30）,示差折光检测器.结果 蔗果三糖浓度在0.3050～6.100mg·mL-1范围内与峰面积呈良好的线性关系,r为0.9999;蔗果四糖浓度在0.4054～8.108mg·mL-1范围内与峰面积呈良好的线性关系,r为0.9999;蔗果五糖浓度在0.2457～4.914mg·mL-1范围内与峰面积呈良好的线性关系,r为0.9996;蔗果三糖加样回收率的平均值为96.1%,RSD=1.5%;蔗果四糖加样回收率的平均值为100.3%,RSD=2.9%;蔗果五糖加样回收率的平均值为100.0%,RSD=3.3%.结论 本方法简便、准确可靠,适用于奶粉中蔗果三糖、蔗果四糖和蔗果五糖三个主要有效成分的测定.

OBJECTIVE To develop a method for determination of 1-Kestose, Nystose and 1F-Fructofuranosytnystose in milk powder by HPLC. METHODS The chromatographic separation was achieved on Aglient NH2 column （ 250mm × 4.6mm,5μm） with the mobile phase consisted of acetonitrile and water （ 70：30 ） and refractive index detector. RESULTS The calibration curves were linear in the range of 0. 3050 × 6. 100mg ·mL-1 for 1-Kestose（ r = 0. 9999） ;The calibration curves were linear in the range of 0. 4054 - 8. 108mg · mL-1 for Nystose（ r = 0. 9999） ; the calibration curves were linear in the range of 0. 2457 - 4. 914mg· mL-1 for 1F-Fructofuranosylnystose （ r = 0. 9996）. The average recoveries of 1-Kestose were 96. 1% （ RSD = 1.5 % ） ;The average recoveries of Nystose were 100. 3% （ RSD = 2. 9% ） ; The average recoveries of 1F-Fructofuranosylnystose were 100. 0% （ RSD = 3.3% ） . CONCLUSIONS The method is simple, accurate, reliable and can be used for the quality control of 1-Kestose, Nystose and 1F-Fructofuranosylnystose in milk powder.