转染Zbtb7a基因对人胃癌SGC7901细胞增殖、凋亡的影响及其机制

Effect of Zbtb7a gene transfer on proliferation and apoptosis of gastric cancer cells SGC7901

目的观察转染Zbtb7a基因对人胃癌细胞系SGC7901增殖及凋亡的影响,并探讨其机制。方法将pcDNA3.1-Zbtb7a和pSilencer 3.1-H1-mk用Lipofectamine2000转染SGC7901细胞,采用RT-PCR和Western Blot法检测MK mRNA及蛋白表达,CCK-8试剂盒和平板克隆法检测细胞增殖,流式细胞仪Annexin V-PI染色检测细胞凋亡。结果转染Zbtb7a后,SGC7901细胞中MK表达水平明显升高,细胞增殖能力明显增强(P<0.05),并且0.5ng/mL TRAIL诱导的细胞凋亡受到抑制(P<0.05)。Zbtb7a高表达后干扰MK的表达,细胞增殖及克隆能力则明显降低(P<0.05),TRAIL诱导的细胞凋亡数也明显增加(P<0.05)。结论 Zbtb7a可以通过上调MK的表达,促进SGC-7901细胞增殖以及抑制TRAI诱导的细胞凋亡。

Objective To observe the effect of Zbtb7 a gene transfer on the proliferation and apoptosis of human gastric cancer cells SGC7901 and to explore its mechanism.Methods The recombinant plasmids pcDNA3.1-zbtb7a and pSilencer 3.1-H1-mk were transfected into the SGC7901 cells by using Lipofectamine2000.The mRNA and protein expression of MK was determined by RT-PCR and Western blotting,respectively.The cell proliferation was measured by CCK-8 kit and colony formation assay.The apoptosis was detected by flow cytometry using annexin V and propidium iodide staining.Results After Zbtb7a gene transfer,the expression of MK and cell proliferation of SGC7901 cells were enhanced,and 0.5 ng/mL TRAIL-induced apoptosis was inhibited （all P 〈 0.05）.The high expression of Zbtb7a gene interfered the MK expression,which decreased the cell proliferation and enhanced the 0.5 ng/mL TRAIL-induced apoptosis （all P 〈0.05）.Conclusion Zbtb7a can promote proliferation and suppress TRAIL-induced apoptosis of human gastric cancer cells SGC7901 by up-regulating MK expression.