兴安柴胡化学成分研究(Ⅱ)

Studies on the Chemical Components of Bupleurum Sibiricum Vest(Ⅱ)

从兴安柴胡根部乙醚提取部分通过层析方法分得5种化合物,经光谱方法鉴定为6,7,3′,8′-双藁本内酯(B_1),6,7,3a′,6′-双藁本内酯(B_2),顺-6,7-二羟基藁本内酯(B_3),孕甾烯醇酮(B_4),香草醛(B_5),其中B_1为新化合物。

Five compounds, B1, B2, B3, B4, B5 were obtained from the ether extract of Bupleu-rum Sibiricum Vest by means of silica gel, alumina and Sephadex LH-20 column chroma-tographies. The structures were elucidated on the basis of spectral analyses (IR, UV, CD, EIMS, 'H NMR and 13C NMR, DEPT, 'H-'H COSY, 'H-'H NOESY and single crystal X-ray analysis) and chemical reactions. The compounds were identified as : Z,Z'-6, 7,3' ,8'-dili-gustilide(B1 ) , Z, Z'-6, 7, 3a', 6'-diligustilide (B2), cis-6, 7-dihydroxyligustilide (B3), preg-nenolone(B4) and vanillin(B5). Compound Bj is a new compound. The others four, B2,B3,B4, B5 were isolated from the Bupleurum genus for the first time.