斑蝥复方药酒提物对小鼠肝癌H-22的治疗作用研究

Compound cantharidin tincture extract of mice of the treatment of liver cancer H-22

目的通过体内外实验观察斑蝥复方药的抗肿瘤作用。方法①体内实验:建立小鼠肝癌荷瘤动物模型,通过灌胃给药的方法,检测原位肿瘤、免疫器官指数、以及血清中Na+、K+、Cl-离子、葡萄糖等活性;评价斑蝥复方药物的抗肿瘤作用。②体外实验:培养大肠癌细胞与小鼠脾细胞,用MTT法测定药物对大肠癌细胞的毒性作用和对小鼠脾细胞的增殖抑制作用。根据实验综合评价其抗肿瘤的作用。结果体内试验在本实验剂量下,与模型对照组比较,治疗组有不同程度的肿瘤生长减慢,抑瘤率为27.7%,显著降低胸腺指数增加肝指数。并非常显著的提高血清中钠离子和氯离子的浓度,一定浓度的斑蝥复方药对大肠癌细胞有毒性作用,且对小鼠脾淋巴细胞增殖不明显。结论斑蝥复方药有抗肿瘤作用,且对机体产生一定的毒性,并对体内的电解质及其代谢有一定的影响。

Objective To observe the cantharidin vivo anti-tumor effect of the drug compound. Methods （1 vivo： establish a mouse model of liver tumor-bearing animals by intragastric administration method, in situ detection of cancer, immune organ index, and serum Na, K, Cl ions, glucose and other activity; evaluation of compound cantharidin antitumor drugs. （2） In vitro： cultured colon cancer cells and mouse spleen cells by MTT method of drug toxicity in colorectal cancer ceils and proliferation of mouse spleen cells. According to the experimental evaluation of its role. Results The in vivo doses in this study, compared with the model group, treatment group, tumor growth slowed down in varying degrees, inhibitory rate was 27.7%, the measured tumor-bearing mice treated group had significant organ index difference. A certain concentration of cantharidin drug compound HCT-116 colon cancer cell toxic effects, and on lymphocyte proliferation to some extent. Conclusion cantharidin drugs on cancer cells HCT- 116 of the IC50 was 0. 114 micrograms / ml, the IC50 of mouse spleen lymphocytes was 1. 958 micrograms / mlConclusion cantharidin drug compound has anti-tumor effect, and on the body to produce some toxicity, and metabolism of the body＇s electrolytes and to a certain extent.