电针次髎/中髎穴抑制膀胱过度活动的穴位特异性研究

Electroacupuncture at BL32 /BL33 in the inhibition of overactive bladder and its acupoints specificity

目的探讨次髎和中髎穴抑制膀胱过度活动的穴位特异性。方法 60只SD大鼠随机分为模型组和5个电针组:次髎/中髎组、委中组、三阴交组、通天组、合谷组,每组10只。各组大鼠均先采用生理盐水进行膀胱灌注(基线Ⅰ),继以0.25%乙酸膀胱灌注建立大鼠膀胱过度活动模型(基线Ⅱ)。造模成功后,5个电针组进行电针治疗5分钟,模型组不予电针治疗。电针后再次以生理盐水进行膀胱灌注。观察灌注期间大鼠排尿间隔时间、膀胱排尿压力、膀胱基础压力和平均排尿压力的改变。结果与基线Ⅰ相比,基线Ⅱ时各组排尿间隔时间显著缩短(P<0.01)、膀胱排尿压力和平均排尿压力显著下降(P<0.01)。电针干预后,各组排尿间隔时间较基线Ⅱ均显著延长,仅次髎/中髎组与基线Ⅰ比较差异不显著,并且其增加值显著大于模型组(P<0.001)和其他电针组(P<0.01,或P<0.05)。次髎/中髎组电针后膀胱排尿压力较基线Ⅱ显著升高(P<0.05)、与基线Ⅰ差异不显著,并显著高于模型组(P<0.001)和其他电针组(P<0.05)。结论次髎、中髎可有效抑制膀胱过度活动,此抑制效应具有穴位特异性。

Objective To investigate the acupoints specificity of Ciliao（ BL32） and Zhongliao（ BL33） on inhibiting overactive activity of bladder. Methods 60 SD rats were randomly divided into 1 model group and 5 electroacupuncture groups： Ciliao / Zhongliao（ BL32 / BL33） group,Weizhong（ BL40） group,Hegu（ LI4） group,Tongtian（ BL7） group and Sanyinjiao（ SP6） group. Rats in each group were performed with bladder perfusion with normal saline（ basalⅠ,to get the normal data of rats） and 0. 25% Acetic acid（ basalⅡ,to make rat model of overactive bladder）,respectively. Rats in 5 electroacupunc- ture groups received elecltroacupuncture treatment 5 minutes,while rats in model group received no inter- vention. Rats in each group were performed with bladder perfusion with normal saline again. Changes of in- tercontraction interval（ ICI）,vesical micturition pressure（ VMP）,basal pressure and average voiding pressure in all groups were observed. Results Compared with basalⅠ,the ICI of each group was signifi- cantly shortened（ P〈0. 01） and the VMP significantly decreased（ P〈0. 01） at basalⅡ. After electroa- cupuncture treatment,the ICI were prolonged in all groups but also shorter than basal Ⅰ except Ciliao / Zhongliao group,while the increased value of Ciliao / Zhongliao group significantly greater than model group（ P〈0. 001） and other electroacupuncture groups（ P〈0. 01 or P〈0. 05）. The VMP of Ciliao/Zhongliao group was significantly increased（ P〈0. 05） compared with the model group（ P〈0. 001） and other elec- troacupuncture groups（ P〈0. 05）. Conclusion Electroacupuncture at Ciliao / Zhongliao point can inhibit bladder hyperactivity,which shows an effect of acupoints specificity.