运动训练结合中医外治疗法对大鼠缺血下肢骨骼肌毛细血管密度的影响

Effects of exercise training combined with external therapy of traditional Chinese medicine on capillary density in rat skeletal muscle ischemia of lower extremities

目的观察运动训练结合中医外治(动脉2号方)疗法对大鼠缺血下肢骨骼肌毛细血管密度的影响。方法 SD大鼠90只随机分为模型组、运动组、外治组、运动﹢外治组、正常组,每组18只。除正常组外,余组建立大鼠下肢缺血模型,术后3天开始治疗,术后10天、17天、21天,大鼠外周血酶测定、微血管密度(MVD)测定、HE病理染色,比较各组差异。结果术后10天、17天、21天,运动+外治组血酶值均最低,术后17天、21天运动+外治组MVD值显著高于其他组(P<0.01)。结论运动训练结合中医外治(动脉2号方)疗法可以改善下肢缺血,促进大鼠下肢缺血模型的毛细血管密度的增加,促进血管新生。

Objective To observe the effects of therapy of exercise combined with traditional Chi- nese medicine（ artery No. 2） effects of therapy on capillary density of rat skeletal muscle ischemia of lower extremities. Methods 90 SD rats were randomly divided into model group,exercise group,treatment group,exercise ＋ external treatment group,the normal group,18 rats in each group. Except the normal group,the model of lower limb ischemia rats sets,treatment was started 3 days after surgery,after 10 days,17 days,21 days,serum enzyme determination,rat microvessel density（ MVD） were determined, HE pathological staining. compared the difference. Results After 10 days,17 days,21 days,sports and external treatment group blood enzyme values were the lowest,17 days after operation,21 days of exercise ＋ external treatment group MVD was significantly higher than other groups（ P 0. 01）. Conclusion Ex- ternal treatment of TCM combined with exercise training（ artery No. 2） therapy can improve the lower limb ischemia,increase lower limb ischemia in the rat model of capillary density,angiogenesis.