推拿对坐骨神经损伤大鼠运动功能和神经丝蛋白-M的影响

Effect of Tuina therapy on motor function and scaffolding proteins NF-M in neurons of sciatic nerve injury rat models

目的从坐骨神经损伤大鼠行为学和神经丝蛋白M（NF-M）的角度,探讨推拿治疗坐骨神经损伤的起效机制。方法采用夹持法建立坐骨神经损伤模型,将大鼠随机分为正常组、假手术组、模型组、模型对照组、推拿组,以按摩推拿手法模拟仪进行干预,通过斜板实验观察各组大鼠行为学变化;通过免疫组织化学染色观察各组大鼠L3~L5脊髓内NF-M表达情况。结果模型组、模型对照组大鼠斜板实验评分与正常组比较均有明显降低,推拿治疗后斜板实验评分与模型组比较有明显提高,并在治疗20天后与正常组无显著性差异（P〉0.05）;模型组、模型对照组及推拿组NF-M免疫组化表达与正常组比较均有明显提高,推拿组与模型组比较有显著性差异（P〈0.05）。结论推拿治疗坐骨神经损伤可能是通过提高神经元骨架蛋白NF-M在脊髓内的表达,从而促进轴浆运输功能的恢复,促进神经元的存活,最终改善坐骨神经损伤大鼠的运动功能。

Objective To discuss the principal mechanisms for tuina therapy on Sciatic Nerve In- jury from the viewpoint of behavioristics and scaffolding proteins NF-M in neurons of Sciatic Nerve Injury rat models. Methods Use the clamping method to induce the sciatic nerve injury model. And then to observe the behavioral changes by oblique board test and the expression of scaffolding proteins NF-M in L3-L5 spi- nal cord by immunohistochemical staining in normal group,sham-operated group,model group,model con- trol group and Tuina group rats intervened with massage simulator. Results Inclined plate test scores of model group,model control group rats compared with that of the normal group were significantly lower. After being treated for 20 days（ P〈0. 05）,compared with normal group there is no significant differences. Model group,model control group and the massage group＇s immunohistochemical expressions of NF-M compared with that of the normal group were significantly increased,and there is statistically significant difference between massage group and model group（ P〈0. 05）. Conclusion The massage therapy promote the axoplasmic transport function,accelerate neuronal survival and ultimately improve the motor function of sciatic nerve injury model rats by the expression of scaffolding proteins NF-M in spinal cord.