摘要
建立用高效液相色谱法测定冬虫夏草中虫草酸含量的方法。WatersCarbohydrateHighperformance（4．6mm×250mm，4um）；70％（v／V）乙腈为流动相；流速1．0mL／min，柱温40℃，示差折光检测器，检测器温度40℃。线性范围0．986—12．325ug，r=0．9999；加标回收率为100．9％。该法简便、快速、稳定性好；可作为冬虫夏草中虫草酸的检测方法。
To establish a HPLC method for determining the contents of cordycepic acid in Cordyceps. The cordycepic acid were separated on a Waters Carbohydrate High performance column （4.6 mm ×250 mm,4 um） ,with 70% （V/V） acetonitrile solution as mobile phase and detected with a refractive index detector system held in the oven of 40℃. The calibration curve was linear in the range of 0. 986 ~ 12. 325 ug （ r = 0. 9999 ） for cordycepic acid. The recovery of stand- ard addition was 100.9% The method is simple, rapid and stable. It could be used to determine the contents of cordyce- pic acid in Cordyceps.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第B12期68-71,共4页
Natural Product Research and Development
基金
基金项目：国家863项目（2010AA023005）
